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Multiplex Detection of Fusarium Species

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Mycotoxigenic Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1542))

Abstract

Multiplex PCR is a powerful method to detect, identify, and quantify the mycotoxigenic fungus by targeting the amplification of genes associated with mycotoxin production and detection, identification, and quantification of Fusarium species. As compared with uniplex PCR, it has several advantages such as low cost, shortened time, and simultaneous amplification of more than two genes (in only one reaction tube). Here, we describe multiplex PCR-based detection and identification of trichothecene-, zearalenone-, fumonisin-, and enniatin-producing Fusarium species, the use of multiplex PCR in multiplex genotype assay and the use of multiplex TaqMan real-time qPCR.

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Acknowledgements

This work was supported by the Academy of Finland (no. 52104), National Technology Agency of Finland (No. 40168/03), and the Nordic Research Board (No. 040291).

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Authors and Affiliations

  1. Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20520, Turku, Finland

    Tapani Yli-Mattila

  2. DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, India

    Siddaiah Chandra Nayaka

  3. Microbiology Division, DRDO-BU-Centre for Life sciences, Bharathiar University Campus, Coimbatore, 641046, TamilNadu, India

    Mudili Venkataramana

  4. Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Yeni Yuzyil University, 34010, Istanbul, Turkey

    Emre Yörük

Authors
  1. Tapani Yli-Mattila
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  2. Siddaiah Chandra Nayaka
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  3. Mudili Venkataramana
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  4. Emre Yörük
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Corresponding author

Correspondence to Tapani Yli-Mattila .

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Editors and Affiliations

  1. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonio Moretti

  2. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonia Susca

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Yli-Mattila, T., Nayaka, S.C., Venkataramana, M., Yörük, E. (2017). Multiplex Detection of Fusarium Species. In: Moretti, A., Susca, A. (eds) Mycotoxigenic Fungi. Methods in Molecular Biology, vol 1542. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6707-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6707-0_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6705-6

  • Online ISBN: 978-1-4939-6707-0

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