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Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND)

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Cancer Cytogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1541))

Abstract

Multicolor fluorescence in situ hybridization (mFISH) approaches are routine applications in tumor as well as clinical cytogenetics nowadays. The first approach when thinking about mFISH is multicolor karyotyping using human whole chromosome paints as probes; this can be achieved by narrow-band filter-based multiplex-FISH (M-FISH) or interferometer/spectroscopy-based spectral karyotyping (SKY). Besides, various FISH-based banding approaches were reported in the literature, including multicolor banding (MCB/mBAND) the latter being evaluated by narrow-band filters, and using specific software. Here, we describe the combined application of multicolor karyotyping and MCB/mBAND for the characterization of simple and complex acquired chromosomal changes in cancer cytogenetics.

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Acknowledgments

Supported in parts by the Wilhelm Sander Stiftung (2013.032.1) and German academic exchange service (DAAD).

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Authors and Affiliations

  1. Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743, Jena, Germany

    Thomas Liehr, Moneeb A. K. Othman & Katharina Rittscher

Authors
  1. Thomas Liehr
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  2. Moneeb A. K. Othman
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  3. Katharina Rittscher
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Corresponding author

Correspondence to Thomas Liehr .

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Editors and Affiliations

  1. Haematology Division Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong Prince of Wales Hospital, Shatin, Hong Kong, China

    Thomas S.K. Wan

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Liehr, T., Othman, M.A.K., Rittscher, K. (2017). Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND). In: Wan, T. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 1541. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6703-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6703-2_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6701-8

  • Online ISBN: 978-1-4939-6703-2

  • eBook Packages: Springer Protocols

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