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Hydrodynamic HBV Transfection Mouse Model

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Hepatitis B Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1540))

Abstract

Hydrodynamic HBV transfection mouse model is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.

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Acknowledgment

This work was supported by Ministry of Science of Technology (MOST) grant for the advancement of science and technology.

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Authors and Affiliations

  1. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan

    Li-Ling Wu, Hurng-Yi Wang & Pei-Jer Chen

Authors
  1. Li-Ling Wu
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  2. Hurng-Yi Wang
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  3. Pei-Jer Chen
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Corresponding author

Correspondence to Pei-Jer Chen .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA

    Haitao Guo

  2. Arbutus Biopharma, Inc., Doylestown, Pennsylvania, USA

    Andrea Cuconati

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Wu, LL., Wang, HY., Chen, PJ. (2017). Hydrodynamic HBV Transfection Mouse Model. In: Guo, H., Cuconati, A. (eds) Hepatitis B Virus. Methods in Molecular Biology, vol 1540. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6700-1_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6700-1_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6698-1

  • Online ISBN: 978-1-4939-6700-1

  • eBook Packages: Springer Protocols

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