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Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications

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Hepatitis B Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1540))

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Abstract

Measurement of secreted HBV antigens in cell culture is an important endpoint in many experimental settings. Here we describe convenient and inexpensive protocols for 96-well format sandwich ELISA assays for this purpose, in which there are many options for customization of antibodies used and other parameters. These protocols can be adapted to use for animal serum samples, compound library screening, and other purposes.

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Author information

Authors and Affiliations

  1. Arbutus Biopharma, Inc., 3805 Old Easton Road, Doylestown, PA, 18902, USA

    Xiaohe Wang & Andrea Cuconati Ph.D.

  2. Baruch S. Blumberg Institute, 3805 Old Easton Road, Doylestown, PA, 18902, USA

    Cally D. Goddard & Lale Bildrici-Ertekin

Authors
  1. Cally D. Goddard
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  2. Lale Bildrici-Ertekin
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  3. Xiaohe Wang
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  4. Andrea Cuconati Ph.D.
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Corresponding author

Correspondence to Andrea Cuconati Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA

    Haitao Guo

  2. Arbutus Biopharma, Inc., Doylestown, Pennsylvania, USA

    Andrea Cuconati

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Goddard, C.D., Bildrici-Ertekin, L., Wang, X., Cuconati, A. (2017). Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications. In: Guo, H., Cuconati, A. (eds) Hepatitis B Virus. Methods in Molecular Biology, vol 1540. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6700-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6700-1_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6698-1

  • Online ISBN: 978-1-4939-6700-1

  • eBook Packages: Springer Protocols

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