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Oral Biology pp 79-105 | Cite as

NMR-Based Metabolomics of Oral Biofluids

  • Horst Joachim SchirraEmail author
  • Pauline J. Ford
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1537)

Abstract

NMR-based metabolomics is an established technique for characterizing the metabolite profile of biological fluids and investigating how metabolite profiles change in response to biological and/or clinical stimuli. Thus, NMR-based metabolomics has the potential to discover biomarkers for diagnosis, prognosis, and/or therapy of clinical conditions, as well as to unravel the physiology underlying clinical conditions. Here, we describe a detailed protocol for NMR-based metabolomics of oral biofluids, including sample collection, sample handling, NMR data acquisition, and processing. In addition, we give a general overview of the statistical analysis of the resulting metabolomic data.

Key words

Metabolomics Systems biology NMR spectroscopy Saliva Gingival crevicular fluid 

Notes

Acknowledgments

We gratefully acknowledge Dr Shaneen Leishman for assistance in refining the collection methods. We are grateful to Dr Emma Broughton and Dr Rachel Dunn for preparing and analyzing the saliva and GCF samples used for Fig. 2. NMR spectra for Fig. 2 were measured at the University of Queensland’s 900 MHz spectrometer, which is part of the Queensland NMR Network (QNN), and the authors acknowledge financial support provided by the Queensland State Government to the Queensland NMR Network facilities at The University of Queensland. We wish to thank Dr Gregory Pierens for critical reading of the manuscript and helpful advice.

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Centre for Advanced Imaging, The University of QueenslandBrisbaneAustralia
  2. 2.School of Dentistry, Oral Health CentreThe University of QueenslandHerstonAustralia

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