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Oral Biology pp 481-497 | Cite as

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

  • Phillipa C. White
  • Ilaria J. Chicca
  • Martin R. Ling
  • Helen J. Wright
  • Paul R. Cooper
  • Mike R. Milward
  • Iain L. C. ChappleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1537)

Abstract

Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

Key words

Neutrophil extracellular traps Reactive oxygen species Fluorescence microscopy SEM Chemiluminescence DNA Elastase Myeloperoxidase Cathepsin G Immunostaining 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Phillipa C. White
    • 1
  • Ilaria J. Chicca
    • 1
  • Martin R. Ling
    • 1
  • Helen J. Wright
    • 1
  • Paul R. Cooper
    • 1
  • Mike R. Milward
    • 1
  • Iain L. C. Chapple
    • 1
    Email author
  1. 1.Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of DentistryUniversity of BirminghamBirminghamUK

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