Proteomic Analysis of Dental Tissue Microsamples
Improved understanding of dental enamel development will benefit not only dentistry but also biomedicine more generally. Rat and mouse models of enamel development are relatively well characterized and experimentally powerful. However, the diminutive size of murine teeth makes them difficult to study using standard proteomics approaches. Here, we describe gel-based proteomic methods that enable parallel quantification, identification, and functional characterization of proteins from developing rat and mouse teeth. These refined methods are applicable to other scarce samples including human enamel defects.
Key wordsMicrosample proteomics Dental development Enamel defects Rat and mouse models Ameloblast Sample preparation Gel electrophoresis Functional proteomics
We thank Nicola McHugh for skilfully assisting with development of the 2DGE procedures described here. This work was supported by the Melbourne Research Unit for Facial Disorders, the National Health and Medical Research Council of Australia, and the Health Research Council of New Zealand.