Growing Adipose-Derived Stem Cells Under Serum-Free Conditions
Growing adipose-derived stem cells (ADSC) in serum-free conditions is important as it represents a way of expanding multipotent cells in a clinical grade medium. Most cultured ADSC are expanded and tested in serum-containing media, which can pose significant health risks if these cells were used in clinical applications. Moreover, cells grown in serum-free conditions behave significantly different than those cultured in serum-containing media. Here, we present a technique to culture adipose-derived stem cells in serum-free conditions. The methods described in this chapter were optimized for ovine ADSC. The appropriate optimization should be done for other cell lines.
Key wordsAdipose-derived stem cells (ADSC) Mesenchymal stem cells (MSC) Serum-free media Ovine Fetal bovine serum (FBS)
The financial support provided by grants from the New Zealand Lottery Board (Lottery Health Research Grant) and the Otago Medical Research Foundation (Jack Thompson Arthritis Grant), and a University of Otago Doctoral Scholarship (awarded to D.G.Z.) are gratefully acknowledged. We also thank the Molecular Biosciences Laboratory (Faculty of Dentistry) personnel.
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