Oral Biology pp 429-438 | Cite as

Constructing Tissue Microarrays: Protocols and Methods Considering Potential Advantages and Disadvantages for Downstream Use

  • Lynne BingleEmail author
  • Felipe P. Fonseca
  • Paula M. Farthing
Part of the Methods in Molecular Biology book series (MIMB, volume 1537)


Tissue microarrays were first constructed in the 1980s but were used by only a limited number of researchers for a considerable period of time. In the last 10 years there has been a dramatic increase in the number of publications describing the successful use of tissue microarrays in studies aimed at discovering and validating biomarkers. This, along with the increased availability of both manual and automated microarray builders on the market, has encouraged even greater use of this novel and powerful tool. This chapter describes the basic techniques required to build a tissue microarray using a manual method in order that the theory behind the practical steps can be fully explained. Guidance is given to ensure potential disadvantages of the technique are fully considered.

Key words

Tissue Microarray Immunohistochemistry Candidate Biomarker Validation 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Lynne Bingle
    • 1
    Email author
  • Felipe P. Fonseca
    • 2
  • Paula M. Farthing
    • 1
  1. 1.Academic Unit of Oral and Maxillofacial Pathology, School of Clinical DentistryUniversity of SheffieldSheffieldUK
  2. 2.Department of Oral Diagnosis, Faculty of Dentistry of Piracicaba, FOPUNICAMPPiracicabaBrazil

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