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Oral Biology pp 413–427Cite as

A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1537))

Abstract

Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1). Although no single antibody demonstrates specificity for MSCs, isolation based on expression of individual markers results in homogenous preparations of PDLSCs. Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast-, and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo, are also described.

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Author information

Authors and Affiliations

  1. Colgate Australian Dental Research Centre, Dental School, University of Adelaide, Adelaide, SA, Australia

    Krzysztof Mrozik

  2. Department of Anatomy and Cell BiologySchool of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Songtao Shi

  3. Colgate Australian Clinical Dental Research Centre, Dental School, University of Adelaide, Frome Road, Adelaide, SA, 5005, Australia

    P. Mark Bartold

  4. Mesenchymal Stem Cell Group, Adelaide Medical School, Faculty of Health Sciences, University of Adelaide, Adelaide, Adelaide, SA, 5005, Australia

    Stan Gronthos

Authors
  1. Krzysztof Mrozik
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  2. Stan Gronthos
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  3. Songtao Shi
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  4. P. Mark Bartold
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Editor information

Editors and Affiliations

  1. University of Otago Fac. Dentistry, Dunedin, New Zealand

    Gregory J. Seymour

  2. Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Mary P. Cullinan

  3. Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Nicholas C.K. Heng

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Mrozik, K., Gronthos, S., Shi, S., Bartold, P.M. (2017). A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 1537. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6685-1_24

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  • DOI: https://doi.org/10.1007/978-1-4939-6685-1_24

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6683-7

  • Online ISBN: 978-1-4939-6685-1

  • eBook Packages: Springer Protocols

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