Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease
The interactions between bacteria, epithelium, and neutrophilic polymorphonuclear leukocytes (neutrophils) are the key to the initiation and progression of many chronic inflammatory-immune diseases. In addition, all can be influenced by external factors, such as micronutrients, thereby providing potentially novel approaches to therapy. This chapter will therefore provide detailed methods for core techniques involved in studying cellular and molecular epithelial responses to a bacterial challenge in relation to chronic inflammatory disease pathogenesis and therapy.
Key wordsEpithelium Immunocytochemistry Gene expression Polymerase chain reaction Electrophoresis H400 oral epithelial cells NF-kappa B DNA RNA
The methods and diagrams are adapted and updated from “Oral Epithelium in the Pathogenesis of Periodontitis” Ph.D. by Michael R Milward (2010). High content analysis was performed in conjunction with Imagen Biotech, UK