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Oral Biology pp 367-380 | Cite as

Embryonic Explant Culture: Studying Effects of Regulatory Molecules on Gene Expression in Craniofacial Tissues

  • Katja NärhiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1537)

Abstract

The ex vivo culture of embryonic tissue explants permits the continuous monitoring of growth and morphogenesis at specific embryonic stages. The functions of soluble regulatory molecules can be analyzed by introducing them into culture medium or locally with beads to the tissue. Gene expression in the manipulated tissue explants can be analyzed using in situ hybridization, quantitative PCR, and reporter constructs combined to organ culture to examine the functions of the signaling molecules.

Key words

Mouse Morphogenesis Signaling molecule Organ culture Tooth Whisker Palate Calvarial bone In situ hybridization Real-time quantitative PCR 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Institute for Molecular Medicine FinlandUniversity of HelsinkiHelsinkiFinland

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