Oral Biology pp 191-202 | Cite as

Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)

  • Mª José Marin
  • Elena Figuero
  • David Herrera
  • Mariano SanzEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1537)


The quantitative polymerase chain reaction (qPCR) is a variant of PCR aimed to detect and quantify a targeted DNA molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated PCR product. This chapter presents the detailed procedures for quantification of different periodontal pathogens (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, and Fusobacterium spp.) using qPCR. It also includes the description of the most frequent problems encountered and how to solve them. In addition, a detailed protocol for multiplex qPCR to detect and quantify P. gingivalis and A. actinomycetemcomitans is included.

Key words

Quantitative PCR (qPCR) TaqMan Primers Probe Multiplex qPCR Aggregatibacter actinomycetemcomitans Porphyromonas gingivalis Tannerella forsythia Campylobacter rectus Fusobacterium spp 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Mª José Marin
    • 1
  • Elena Figuero
    • 1
    • 2
    • 3
  • David Herrera
    • 2
    • 3
  • Mariano Sanz
    • 2
    • 3
    Email author
  1. 1.Oral Research Laboratory, Faculty of OdontologyUniversity ComplutenseMadridSpain
  2. 2.Etiology and Therapy of Periodontal Diseases (ETEP) Research GroupUniversity ComplutenseMadridSpain
  3. 3.Department of Periodontology, Faculty of DentistryUniversity Complutense of MadridMadridSpain

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