Abstract
This protocol specifies an accurate and sensitive method for the determination of T-2 and HT-2 toxins content in oats and oat-based foods using liquid chromatography with tandem mass spectrometry detection. T-2 and HT-2 toxins are extracted from the test material with a mixture of acetonitrile and water. The filtered extract is dried, reconstituted with a mixture of methanol and water, then purified on a polymeric solid-phase extraction cartridge. Toxins are finally eluted from the column with methanol and quantified by reversed phase high performance liquid chromatography with tandem mass spectrometry detection.
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Acknowledgements
The research leading to these results has received funding by the UK Food Standards Agency (Project Number C03056).
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Lattanzio, V.M.T., Pascale, M. (2017). Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry. In: Gasparis, S. (eds) Oat. Methods in Molecular Biology, vol 1536. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6682-0_9
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DOI: https://doi.org/10.1007/978-1-4939-6682-0_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6680-6
Online ISBN: 978-1-4939-6682-0
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