Abstract
Wide hybridization is a one of the important techniques in plant breeding. Oat (Avena sativa L.) and pearl millet (Pennisetum glaucum L.) belong to different subfamilies of Poaceae. In generally, such distant relative species show uniparental chromosome elimination after successful fertilization. However, all seven pearl millet chromosomes are retained beside the genome of oat during embryogenesis. Hybrid seedlings develop, but show necrosis after light irradiation. Here, a detailed protocol for wide hybridization between oat and pearl millet is described.
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I would like to thank Dr. Andreas Houben, IPK, Germany, for reading and providing valuable comments on this manuscript.
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Ishii, T. (2017). Wide Hybridization Between Oat and Pearl Millet. In: Gasparis, S. (eds) Oat. Methods in Molecular Biology, vol 1536. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6682-0_3
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DOI: https://doi.org/10.1007/978-1-4939-6682-0_3
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