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The Zebrafish as a Model for Human Bacterial Infections

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Bacterial Pathogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1535))

Abstract

The development of the zebrafish (Danio rerio) infectious disease model has provided new insights and information into pathogenesis. Many of these new discoveries would not have been possible using a typical mammalian model. The advantages of using this model are many and in the last 15 years the model has been exploited for the analysis of many different pathogens. Here, we describe in detail how to perform a bacterial infection using either the adult zebrafish or zebrafish larvae using microinjection. Multiple methods of analysis are described that can be used to address specific questions pertaining to disease progression and the interactions with the immune system.

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Author information

Authors and Affiliations

  1. Department of Biology, Texas Woman’s University, 1200 Frame St., P.O. Box 425799, Denton, TX, 76204, USA

    Melody N. Neely

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  1. Melody N. Neely
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Corresponding author

Correspondence to Melody N. Neely .

Editor information

Editors and Affiliations

  1. Department of Clinical Sciences, Lund University, Division of Infection Medicine, Lund, Sweden

    Pontus Nordenfelt

  2. Department of Clinical Sciences, Lund University, Division of Infection Medicine, Lund, Sweden

    Mattias Collin

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Neely, M.N. (2017). The Zebrafish as a Model for Human Bacterial Infections. In: Nordenfelt, P., Collin, M. (eds) Bacterial Pathogenesis. Methods in Molecular Biology, vol 1535. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6673-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6673-8_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6671-4

  • Online ISBN: 978-1-4939-6673-8

  • eBook Packages: Springer Protocols

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