Abstract
Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.
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Bottomley, A.L., Turnbull, L., Whitchurch, C.B., Harry, E.J. (2017). Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy. In: Nordenfelt, P., Collin, M. (eds) Bacterial Pathogenesis. Methods in Molecular Biology, vol 1535. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6673-8_12
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DOI: https://doi.org/10.1007/978-1-4939-6673-8_12
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6673-8
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