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Oncogene-Induced Senescence pp 155–163Cite as

Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1534))

Abstract

Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.

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Acknowledgments

The work was financially supported by the DFG Excellence Cluster “Inflammation at Interfaces” and the “Doktor Robert Pfleger Stiftung Bamberg.” D. Zapf is acknowledged for technical assistance.

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Authors and Affiliations

  1. Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany

    Claudia Gey & Karsten Seeger

Authors
  1. Claudia Gey
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  2. Karsten Seeger
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Corresponding author

Correspondence to Karsten Seeger .

Editor information

Editors and Affiliations

  1. Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York, USA

    Mikhail A. Nikiforov

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Gey, C., Seeger, K. (2017). Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence. In: Nikiforov, M. (eds) Oncogene-Induced Senescence. Methods in Molecular Biology, vol 1534. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6670-7_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6670-7_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6668-4

  • Online ISBN: 978-1-4939-6670-7

  • eBook Packages: Springer Protocols

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