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Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells

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Book cover Oncogene-Induced Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1534))

Abstract

The Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.

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Acknowledgment

This work was financially supported by the Bionian Cluster, the Greek GSRT program of Excellence II (Aristeia II, Grant number 3020) and DHI, Medical Group, Athens, Greece.

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Authors and Affiliations

  1. Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greece

    Konstantinos Evangelou & Vassilis G. Gorgoulis

  2. Biomedical Research Foundation, Academy of Athens, Athens, Greece

    Vassilis G. Gorgoulis

  3. Faculty of Biology, Medicine and Health Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK

    Vassilis G. Gorgoulis

Authors
  1. Konstantinos Evangelou
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  2. Vassilis G. Gorgoulis
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Corresponding author

Correspondence to Vassilis G. Gorgoulis .

Editor information

Editors and Affiliations

  1. Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York, USA

    Mikhail A. Nikiforov

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Evangelou, K., Gorgoulis, V.G. (2017). Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. In: Nikiforov, M. (eds) Oncogene-Induced Senescence. Methods in Molecular Biology, vol 1534. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6670-7_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6670-7_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6668-4

  • Online ISBN: 978-1-4939-6670-7

  • eBook Packages: Springer Protocols

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