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Stem Cell-Derived Models in Toxicology pp 271–291Cite as

Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes

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Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Human pluripotent stem cells (hPSC) are investigated as a source of authentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays performed using an automated fluorescence imaging platform and high-content analysis are valuable in characterizing hypertrophic states that may be induced in hPSC-derived cardiomyocytes upon exposure to cardiotoxic compounds. In high-purity populations of hPSC-derived cardiomyocytes loaded with cell tracer probes and other cell markers, detailed hypertrophic profiles can be assessed based on information captured at cellular and subcellular levels.

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Acknowledgements

This work was supported by the UK Medical Research Council core funding to the MRC-UCL University Unit (Ref. MC_EX_G0800785) (JKV), the European Union Seventh Framework Programme (FP7/2007-2013, grant no. PIRG08-GA-2010-276811) (JKV), and the Hungarian Scientific Research Fund (OTKA K-105555) (GF).

Author information

Authors and Affiliations

  1. MRC Laboratory for Molecular Cell Biology, University College London, London, UK

    Janos Kriston-Vizi

  2. National Heart and Lung Institute, Imperial Centre for Experimental and Translational Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK

    Sian E. Harding & Gábor Földes

  3. Heart and Vascular Center, Semmelweis University, Budapest, Hungary

    Gábor Földes

Authors
  1. Janos Kriston-Vizi
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  2. Sian E. Harding
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  3. Gábor Földes
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Corresponding authors

Correspondence to Janos Kriston-Vizi or Gábor Földes .

Editor information

Editors and Affiliations

  1. Axion BioSystems, Inc., Atlanta, Georgia, USA

    Mike Clements

  2. Cell Applications, GE Healthcare, Cardiff, United Kingdom

    Liz Roquemore

Glossary

ANF

Atrial natriuretic peptide

BNP

B-type natriuretic peptide

ESC

Embryonic stem cell

GSK

Glycogen synthase kinase

HCM

Hypertrophic cardiomyopathy

hESC

Human embryonic stem cell

hESC-CM

Human embryonic stem cell-derived cardiomyocyte

hiPSC

Human induced pluripotent stem cell

hiPSC-CM

Human induced pluripotent stem cell-derived cardiomyocyte

hPSC

Human pluripotent stem cell

hPSC-CM

Human pluripotent stem cell-derived cardiomyocyte

MAPK

Mitogen activated protein kinase

NFAT

Nuclear factor of activated T-cells

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Kriston-Vizi, J., Harding, S.E., Földes, G. (2017). Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes. In: Clements, M., Roquemore, L. (eds) Stem Cell-Derived Models in Toxicology. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6661-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6661-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6659-2

  • Online ISBN: 978-1-4939-6661-5

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