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Epstein Barr Virus pp 65–78Cite as

Generation and Infection of Organotypic Cultures with Epstein–Barr Virus

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1532))

Abstract

While numerous model systems are available to study EBV latency in B cells and have contributed greatly to our understanding of the role of these cells in the viral life cycle, models to study the EBV life cycle in epithelial cells in vitro are lacking. Epithelial cells are poorly infected in vitro, and EBV-infected cell lines have not been successfully obtained from epithelial tumors. Recently, we have demonstrated that organotypic cultures of oral keratinocytes can be used as a model to study EBV infection in the epithelial tissue. These “raft” cultures generate a stratified tissue resembling the epithelium seen in vivo with a proliferating basal layer and differentiating suprabasal layers. Here, we describe generation of EBV-infected raft cultures established from primary oral mucosal epithelial cells, which exhibit high levels of productive replication induced by differentiation, as well as methods to analyze EBV infection.

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Acknowledgment

This work was supported by National Institute of Health Research Grant R21 DE021583.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, H107, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA, 17033, USA

    Rachel M. Temple, Craig Meyers & Clare E. Sample Ph.D.

  2. Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

    Rachel M. Temple

  3. The Penn State Hershey Cancer Institute, Hershey, PA, 17033, USA

    Craig Meyers & Clare E. Sample Ph.D.

Authors
  1. Rachel M. Temple
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  2. Craig Meyers
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  3. Clare E. Sample Ph.D.
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Corresponding author

Correspondence to Clare E. Sample Ph.D. .

Editor information

Editors and Affiliations

  1. University of Szeged, Szeged, Hungary

    Janos Minarovits

  2. University of Regensburg, Regensburg, Germany

    Hans Helmut Niller

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Temple, R.M., Meyers, C., Sample, C.E. (2017). Generation and Infection of Organotypic Cultures with Epstein–Barr Virus. In: Minarovits, J., Niller, H. (eds) Epstein Barr Virus. Methods in Molecular Biology, vol 1532. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6655-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6655-4_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6653-0

  • Online ISBN: 978-1-4939-6655-4

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