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An Ultrasensitive Biosensing Platform Employing Acetylcholinesterase and Gold Nanoparticles

  • Dingbin Liu
  • Xiaoyuan Chen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1530)

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a well-known strategy for biomarker detection with a color change, which can be seen by the naked eyes. However, the moderate sensitivity of conventional ELISA limits its applications in many cases where the concentrations of biomarker are very low, such as cancer diagnosis. Here we describe an ultrasensitive colorimetric assay based on acetylcholinesterase (AChE)-catalyzed hydrolysis reaction, whose products trigger the aggregation of gold nanoparticles (AuNPs), causing a distinct color change of the solution from red to purple. This enhanced colorimetric immunoassay offers extremely high sensitivity and specificity. In this study, we employed enterovirus 71 (EV71), the major cause of hand, foot, and mouth disease (HFMD), as a model to evaluate the analytical performance of the plasmonic immunoassay.

Key words

Gold nanoparticles Colorimetric immunoassay Acetylcholinesterase Magnetic beads Signal amplification 

Notes

Acknowledgment

We acknowledge the support from the National Natural Science Foundation of China (Grants 21475066 and 81401463), the Fundamental Research Funds for Central Universities (China), and the Intramural Research Program (IRP) of the National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Collaborative Innovation Center of Chemical Science and EngineeringNankai UniversityTianjinChina
  2. 2.Laboratory of Molecular Imaging and Nanomedicine (LOMIN)National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH)BethesdaUSA

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