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Designing Multicomponent Nanosystems for Rapid Detection of Circulating Tumor Cells

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Cancer Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1530))

Abstract

Detection of circulating tumor cells (CTCs) in the blood circulation holds immense promise as it predicts the overall probability of patient survival. Therefore, CTC-based technologies are gaining prominence as a “liquid biopsy” for cancer diagnostics and prognostics. Here, we describe the design and synthesis of two distinct multicomponent magnetic nanosystems for rapid capture and detection of CTCs. The multifunctional Magneto-Dendrimeric Nano System (MDNS) composed of an anchoring dendrimer that is conjugated to multiple agents such as near infrared (NIR) fluorescent cyanine 5 NHS (Cy5), glutathione (GSH), transferrin (Tf), and iron oxide (Fe3O4) magnetic nanoparticle (MNP) for simultaneous tumor cell-specific affinity, multimodal high resolution confocal imaging, and cell isolation. The second nanosystem is a self-propelled microrocket that is composed of carbon nanotube (CNT), chemically conjugated with targeting ligand such as transferrin on the outer surface and Fe3O4 nanoparticles in the inner surface. The multicomponent nanosystems described here are highly efficient in targeting and isolating cancer cells thus benefiting early diagnosis and therapy of cancer.

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Authors and Affiliations

  1. Maharashtra Institute of Medical Education and Research Medical College, Talegaon Dabhade, Pune, 410507, India

    Shashwat S. Banerjee

  2. Actorius Innovations and Research, 100 NCL Innovation Centre, Pune, 411008, India

    Vrushali Khobragade

  3. MAEER’s Maharashtra Institute of Pharmacy, S.No. 124 MIT Campus, Paud Road, Pune, 411038, India

    Jayant Khandare

Authors
  1. Shashwat S. Banerjee
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  2. Vrushali Khobragade
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  3. Jayant Khandare
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Corresponding author

Correspondence to Jayant Khandare .

Editor information

Editors and Affiliations

  1. School of Applied Sciences, Mount Ida College, Newton, Massachusetts, USA

    Reema Zeineldin

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Banerjee, S.S., Khobragade, V., Khandare, J. (2017). Designing Multicomponent Nanosystems for Rapid Detection of Circulating Tumor Cells. In: Zeineldin, R. (eds) Cancer Nanotechnology. Methods in Molecular Biology, vol 1530. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6646-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6646-2_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6644-8

  • Online ISBN: 978-1-4939-6646-2

  • eBook Packages: Springer Protocols

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