Abstract
Post-translational modifications of histones play essential roles in regulating chromatin structure and function. These are tightly regulated in vivo and there is an intricate cross-talk between different marks as they are recognized by specific reader modules present in a large number of nuclear factors. In order to precisely dissect these processes in vitro native reagents like purified chromatin and histone modifying/remodeling enzymes are required to more accurately reproduce physiological conditions. The vast majority of these enzymes need to be part of stable multiprotein complexes with cofactors enabling them to act on chromatin substrates and/or read specific histone marks. In the accompanying chapter, we have described the protocol for purification of native chromatin from yeast cells (Chapter 3). Here, we present the methods to obtain highly purified native chromatin modifying complexes from Saccharomyces cerevisiae, based on Tandem Affinity Purification (TAP). We also present possible applications and useful functional assays that can be performed using these yeast native reagents.
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Acknowledgments
We are grateful to Rhea Utley for significant corrections to this manuscript. Leslie Mitchel and Kristin Baetz for their original short TAP protocol on magnetic beads. This work was supported by a grant from the Canadian Institutes of Health Research (CIHR; MOP-14308). N.L. was supported by a Canadian Government Foreign Affairs/International Trade studentship. J.C. holds the Canada Research Chair in Chromatin Biology and Molecular Epigenetics.
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Lacoste, N., Bhat, W., Côté, J. (2017). Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays. In: Guillemette, B., Gaudreau, L. (eds) Histones. Methods in Molecular Biology, vol 1528. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6630-1_4
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DOI: https://doi.org/10.1007/978-1-4939-6630-1_4
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