Abstract
The identification of a vast array of posttranslational modifications of histone proteins during cell cycle, repair, replication, and transcription has created the challenge of determining structure-function relationships for individual modifications and combinations of modifications. Some of this information can be gathered from indirect immunofluorescence, where the location and cell cycle relationships can be readily identified. Here we present an immunofluorescence protocol that is adapted for the use in histone modifications.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Markaki Y, Gunkel M, Schermelleh L, Beichmanis S, Neumann J, Heidemann M, Leonhardt H, Eick D, Cremer C, Cremer T (2011) Functional nuclear organization of transcription and DNA replication: a topographical marriage between chromatin domains and the interchromatin compartment. Cold Spring Harb Symp Quant Biol 75:475–492. doi:10.1101/sqb.2010.75.042
Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA (2014) A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Nat Cell Biol 16:345–356
Mazia D, Schatten G, Sale W (1975) Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy. J Cell Biol 66:198–200
Koh CM (2013) Preparation of cells for microscopy using cytospin. Methods Enzymol 533:235–240
Zinner R, Teller K, Versteeg R, Cremer T, Cremer M (2007) Biochemistry meets nuclear architecture: multicolor immuno-FISH for co-localization analysis of chromosome segments and differentially expressed gene loci with various histone methylations. Adv Enzyme Regul 47:223–241
Chozinski TJ, Gagnon LA, Vaughan JC (2014) Twinkle, twinkle little star: photoswitchable fluorophores for super-resolution imaging. FEBS Lett 588:3603–3612
Acknowledgements
Hilmar Strickfaden holds a postdoctoral fellowship by the Alberta Cancer foundation and was supported by the Bayerische Forschungsallianz. This work was supported by grants from the Canadian Institutes of Health Research and Alberta Cancer Foundation.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Strickfaden, H., Hendzel, M.J. (2017). Immunofluorescence of Histone Proteins. In: Guillemette, B., Gaudreau, L. (eds) Histones. Methods in Molecular Biology, vol 1528. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6630-1_10
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6630-1_10
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6628-8
Online ISBN: 978-1-4939-6630-1
eBook Packages: Springer Protocols