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Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels

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Book cover Hypertension

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1527))

Abstract

Primary culture of vascular smooth muscle cells is an important in vitro model for the dissection of molecular mechanisms related to a specific physiological or pathological response at the cellular level. Cultured cells also provide an excellent model to study cell biology. This chapter describes a user-friendly and practical protocol for isolation of vascular smooth muscle cells from small and large vessels by enzymatic dissociation, which can be applied to vessels from different species, including rodents and humans.

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References

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Author information

Authors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK

    Augusto C. Montezano, Rheure A. M. Lopes, Karla B. Neves & Francisco Rios

  2. Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada

    Rhian M. Touyz

  3. Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK

    Rhian M. Touyz

Authors
  1. Augusto C. Montezano
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  2. Rheure A. M. Lopes
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  3. Karla B. Neves
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  4. Francisco Rios
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  5. Rhian M. Touyz
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Corresponding author

Correspondence to Augusto C. Montezano .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  2. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

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© 2017 Springer Science+Business Media LLC

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Montezano, A.C., Lopes, R.A.M., Neves, K.B., Rios, F., Touyz, R.M. (2017). Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_27

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  • DOI: https://doi.org/10.1007/978-1-4939-6625-7_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6623-3

  • Online ISBN: 978-1-4939-6625-7

  • eBook Packages: Springer Protocols

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