Hypertension pp 311-320 | Cite as

Isolation and Differentiation of Human Macrophages

  • Francisco J. Rios
  • Rhian M. Touyz
  • Augusto C. Montezano
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1527)

Abstract

Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor—GM-CSF or colony-stimulating factor—M-CSF) or by different cell culture medium concentration. We also describe how to indentify different macrophage activation phenotypes.

Key words

Macrophage Monocyte Human Culture GM-CSF M-CSF 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Francisco J. Rios
    • 1
  • Rhian M. Touyz
    • 2
    • 1
  • Augusto C. Montezano
    • 1
  1. 1.Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research CentreUniversity of GlasgowGlasgowUK
  2. 2.Kidney Research Centre, Department of Medicine, Ottawa Hospital Research InstituteUniversity of OttawaOttawaCanada

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