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Synchronization of Pathogenic Protozoans

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Cell Cycle Synchronization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1524))

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Abstract

Protozoans are single-celled eukaryotes and many of the best-studied protozoans are parasitic to humans (e.g., Plasmodium falciparum causing malaria and Trypanosoma brucei causing sleeping sickness). These organisms are distantly related to humans but with retained eukaryotic type of cellular processes, making them good model systems for studies of the evolution of basic processes like the cell cycle. Giardia intestinalis causes 250 million cases of diarrhea yearly and is one of the earliest diverging protozoans. It is possible to synchronize its cell cycle using compounds that inhibit different steps of the cell cycle and the detailed protocol is described here.

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Author information

Authors and Affiliations

  1. Department of Cell and Molecular Biology, Uppsala University, Box 596, SE-75421, Uppsala, Sweden

    Staffan Svärd

  2. Department of Microbiology, National Veterinary Institute, Uppsala, Sweden

    Karin Troell

Authors
  1. Staffan Svärd
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  2. Karin Troell
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Corresponding author

Correspondence to Staffan Svärd .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary

    Gaspar Banfalvi

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Svärd, S., Troell, K. (2017). Synchronization of Pathogenic Protozoans. In: Banfalvi, G. (eds) Cell Cycle Synchronization. Methods in Molecular Biology, vol 1524. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6603-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6603-5_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6602-8

  • Online ISBN: 978-1-4939-6603-5

  • eBook Packages: Springer Protocols

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