Abstract
Tau is a microtubule binding protein highly abundant in cytoplasm of neurons. However now there is compelling evidence that it is also physiologically released in the extracellular space. Tau is detectable in culture media of various cells, cerebrospinal fluid (CSF), and brain interstitial fluid (ISF). Being able to measure extracellular tau levels in vivo would deep our understanding on the underlying mechanisms of physiological/pathological tau release from neurons. To meet this need, this chapter describes an in vivo microdialysis protocol and materials for the measurement of extracellular tau in ISF from freely moving mice.
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Acknowledgments
The author thanks Dr. David M. Holtzman and Dr. John R. Cirrito for technical suggestions and helpful discussion on the initial tau microdialysis study. This work was supported in part by “Grant-in-Aid for Scientific Research on Innovative Areas (Brain Protein Aging and Dementia Control) (15H01552) from MEXT”.
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Yamada, K. (2017). In Vivo Microdialysis of Brain Interstitial Fluid for the Determination of Extracellular Tau Levels. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_17
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DOI: https://doi.org/10.1007/978-1-4939-6598-4_17
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