Abstract
Technology breakthrough in proteomics enables to gather qualitative and quantitative information about a protein or a complex mixture of proteins. Two-dimensional gel electrophoresis remains an interesting technique, which provides an overview of the complexity of isovariants from a single protein when coupled to western blotting. Here, we describe a detailed protocol for the two-dimensional analysis of microtubule-associated Tau isovariants from cell to human or mouse brain tissue. We provide protocol enabling to separate native and Tau proteins that are aggregated in neurodegenerative disorders such as Alzheimer’s disease.
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Acknowledgments
This work was supported by the Inserm, Lille 2 University, and CNRS. A grant was obtained from ANR, Laboratory of Excellence Labex DISTALZ, and France Alzheimer. The authors declare no conflict of interest.
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Sergeant, N., Fernandez-Gomez, FJ., Obriot, H., Eddarkaoui, S., Buée-Scherrer, V., Buée, L. (2017). Two-Dimensional Electrophoresis Protocols to Analyze the Microtubule-Associated Tau Proteins from Several Biological Sources. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_14
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DOI: https://doi.org/10.1007/978-1-4939-6598-4_14
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