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Liposomes pp 23–47Cite as

Preparation of DRV Liposomes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1522))

Abstract

Dried reconstituted vesicles (DRV) are liposomes that are formulated under mild conditions and have the capability to entrap substantially high amounts of hydrophilic solutes (compared to other types of liposomes). These characteristics make this liposome type ideal for entrapment of labile substances, as peptide, protein, or DNA vaccines, or in general biopharmaceuticals and sensitive drugs. In this chapter, all possible types of DRV liposomes (with respect to the encapsulated molecule characteristics and/or their applications in therapeutics) are introduced, and preparation methodologies (for each type) are described in detail.

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Abbreviations

BisHOP:

1,2-Bis(hexadecylcycloxy)-3-trimethylaminopropane

CD:

Cyclodextrin

CF:

5,6-Carboxyfluorescein

CFA:

Complete Freund's adjuvant

Chol:

Cholesterol

DMPC:

1,2-dimyristoyl-sn-glyceroyl-3-phosphocholine

DC-Chol:

3b-(N,N-Dimethylaminoethane)carbamylcholesterol

DOTAP:

1,2-Dioleyloxy-3-trimethylammonium propane

DOTMA:

N-[1-(2,3-Dioleyloxy) propyl]-N,N,N-triethylammonium

DPPC:

1,2-dipalmitoyl-sn-glyceroyl-3-phosphocholine

DPPE-PEG2000:

1,2-dipalmitoyl-sn-glyceroyl-3-phosphoethanolamine conjugated to polyethylene glycol (MW 2000)

DRV:

Dried rehydrated vesicles, dried reconstituted vesicles

DSPC:

1,2-distearoyl-sn-glyceroyl-3-phosphocholine

HPβ-CD:

Hydroxypropyl-beta-CD

H-PC:

Hydrogenated PC

iv:

Intravenous

MLV:

Multilamellar vesicles

MW:

Molecular weight

PA:

Phosphatidic acid

PB:

Phosphate buffer

PBS:

Phosphate buffered saline

PC:

Phosphatidylcholine

PCS:

Photon correlation spectroscopy

PEG:

Polyethylene glycol

PG:

Phosphatidylglycerol

PRE:

Prednisolone

PS:

Phosphatidylserine

RCM:

Radiographic contrast media

rgp63:

Recombinant glycoprotein of Leishmania

SA:

Stearylamine

SM:

Sphingomyelin

SUV:

Small unilamellar vesicles

Tc:

Lipid transition temperature

TO:

Triolein

USPIOs:

Ultra small paramagnetic iron oxides

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Author information

Authors and Affiliations

  1. Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, 26510, Rio, Patras, Greece

    Sophia G. Antimisiaris

  2. FORTH/ICE-HT, 26510, Rio, Patras, Greece

    Sophia G. Antimisiaris

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  1. Sophia G. Antimisiaris
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Correspondence to Sophia G. Antimisiaris .

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Editors and Affiliations

  1. School of Pharmacy – Boston, MCPHS University, Boston, Massachusetts, USA

    Gerard G.M. D'Souza

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Antimisiaris, S.G. (2017). Preparation of DRV Liposomes. In: D'Souza, G. (eds) Liposomes. Methods in Molecular Biology, vol 1522. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6591-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6591-5_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6589-2

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