Abstract
Chronic pulmonary hypertension (PH) is associated with right ventricular failure and high mortality regardless of the underlying disease. Currently, therapies can improve clinical outcomes in specific subsets of patients, but have little impact on the progression of pulmonary vascular remodeling. Upon new advances in vector development and delivery techniques, gene therapy is a novel strategy in this field with the potential of overcoming the main limitations of approved drug therapies: modulation of novel anti-remodeling targets and selective pulmonary vasculature targeting with minimal systemic effects. In the recent years, several reports have shown that gene transfer to the pulmonary vascular system is feasible in rodent models of PH. Our group has focused on the translation of airway delivery of viral vectors in small and large animals. Here, we describe a procedure to achieve vector transduction at the distal vasculature in animal models of PH and the methods to evaluate the outcomes of this intervention as a promising new approach in pulmonary vascular diseases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Galie N, Hoeper MM, Humbert M, Torbicki A, Vachiery JL, Barbera JA, Beghetti M, Corris P, Gaine S, Gibbs JS, Gomez-Sanchez MA, Jondeau G, Klepetko W, Opitz C, Peacock A, Rubin L, Zellweger M, Simonneau G (2009) Guidelines for the diagnosis and treatment of pulmonary hypertension: the Task Force for the Diagnosis and Treatment of Pulmonary Hypertension of the European Society of Cardiology (ESC) and the European Respiratory Society (ERS), endorsed by the International Society of Heart and Lung Transplantation (ISHLT). Eur Heart J 30(20):2493–2537. doi:10.1093/eurheartj/ehp297, ehp297 [pii]
Lau EMT, Manes A, Celermajer DS, Galiè N (2011) Early detection of pulmonary vascular disease in pulmonary arterial hypertension: time to move forward. Eur Heart J 32:2489. doi:10.1093/eurheartj/ehr160
Vachiery JL, Adir Y, Barbera JA, Champion H, Coghlan JG, Cottin V, De Marco T, Galie N, Ghio S, Gibbs JS, Martinez F, Semigran M, Simonneau G, Wells A, Seeger W (2013) Pulmonary hypertension due to left heart diseases. J Am Coll Cardiol 62(25 Suppl):D100–D108. doi:10.1016/j.jacc.2013.10.033, S0735-1097(13)05876-2 [pii]
Sutendra G, Michelakis ED (2013) Pulmonary arterial hypertension: challenges in translational research and a vision for change. Sci Transl Med 5(208):208sr205. doi:10.1126/scitranslmed.3005428, 5/208/208sr5 [pii]
Ghofrani H-A, D’Armini AM, Grimminger F, Hoeper MM, Jansa P, Kim NH, Mayer E, Simonneau G, Wilkins MR, Fritsch A, Neuser D, Weimann G, Wang C (2013) Riociguat for the treatment of chronic thromboembolic pulmonary hypertension. N Engl J Med 369(4):319–329. doi:10.1056/NEJMoa1209657
Gheorghiade M, Greene SJ, Butler J et al (2015) Effect of vericiguat, a soluble guanylate cyclase stimulator, on natriuretic peptide levels in patients with worsening chronic heart failure and reduced ejection fraction: the socrates-reduced randomized trial. JAMA 314(21):2251–2262
Reynolds PN (2011) Gene therapy for pulmonary hypertension: prospects and challenges. Expert Opin Biol Ther 11(2):133–143. doi:10.1517/14712598.2011.542139
Reynolds AM, Holmes MD, Danilov SM, Reynolds PN (2012) Targeted gene delivery of BMPR2 attenuates pulmonary hypertension. Eur Respir J 39(2):329–343. doi:10.1183/09031936.00187310
Meng L-K, Liu C-G (2010) Gene therapies for pulmonary hypertension—from experimental trials to bedside aspects. Eur J Cardiothorac Surg 37(2):407–419. doi:10.1016/j.ejcts.2009.06.045
Hadri L, Kratlian RG, Benard L, Maron BA, Dorfmuller P, Ladage D, Guignabert C, Ishikawa K, Aguero J, Ibanez B, Turnbull IC, Kohlbrenner E, Liang L, Zsebo K, Humbert M, Hulot JS, Kawase Y, Hajjar RJ, Leopold JA (2013) Therapeutic efficacy of AAV1.SERCA2a in monocrotaline-induced pulmonary arterial hypertension. Circulation 128(5):512–523. doi:10.1161/CIRCULATIONAHA.113.001585,CIRCULATIONAHA.113.001585 [pii]
Granton J, Langleben D, Kutryk MJ, Camack N, Galipeau J, Courtman D, Stewart DJ (2015) Endothelial NO-synthase gene-enhanced progenitor cell therapy for pulmonary arterial hypertension: the PHACeT trial. Circ Res 117:645. doi:10.1161/circresaha.114.305951
Aguero J, Ishikawa K, Hadri L, Santos-Gallego CG, Fish KM, Kohlbrenner E, Hammoudi N, Kho C, Lee A, Ibáñez B, GarcÃa-Alvarez A, Zsebo K, Maron BA, Plataki M, Fuster V, Leopold JA, Hajjar RJ (2016) Intratracheal Gene Delivery of SERCA2a Ameliorates Chronic Post-Capillary Pulmonary Hypertension: A Large Animal Model. J Am Coll Cardiol. 2016;67(17):2032–46. doi:10.1016/j.jacc.2016.02.049
Aguero J, Ishikawa K, Hadri L, Santos-Gallego C, Fish K, Hammoudi N, Chaanine A, Torquato S, Naim C, Ibanez B, Pereda D, Garcia-Alvarez A, Fuster V, Sengupta PP, Leopold JA, Hajjar RJ (2014) Characterization of right ventricular remodeling and failure in a chronic pulmonary hypertension model. Am J Physiol Heart Circ Physiol 307(8):H1204–H1215. doi:10.1152/ajpheart.00246.2014, ajpheart.00246.2014 [pii]
Pereda D, Garcia-Alvarez A, Sanchez-Quintana D, Nuno M, Fernandez-Friera L, Fernandez-Jimenez R, Garcia-Ruiz JM, Sandoval E, Aguero J, Castella M, Hajjar RJ, Fuster V, Ibanez B (2014) Swine model of chronic postcapillary pulmonary hypertension with right ventricular remodeling: long-term characterization by cardiac catheterization, magnetic resonance, and pathology. J Cardiovasc Transl Res 7(5):494–506. doi:10.1007/s12265-014-9564-6
Acknowledgments
This work is supported by NIH P50 HL112324, R01 HL119046, R01 HL117505, R01 HL128099, R01 HL129814, R01HL131404, & T32 HL007824 (R. J. H.), and a Transatlantic Leducq Foundation grant. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health for providing some of the gene vectors used in these studies. J.A. was supported by the Fundacion Alfonso Martin-Escudero. N.H. was supported by the French Federation of Cardiology.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Aguero, J., Hadri, L., Hammoudi, N., Leonardson, L., Hajjar, R.J., Ishikawa, K. (2017). Inhaled Gene Transfer for Pulmonary Circulation. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 1521. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6588-5_24
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6588-5_24
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6586-1
Online ISBN: 978-1-4939-6588-5
eBook Packages: Springer Protocols