Skip to main content
SpringerLink
Log in
Book cover

Cardiac Gene Therapy pp 195–204Cite as

Gene Transfer to Rodent Hearts In Vivo

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1521))

Abstract

Altering expression of specific genes in rodent models allows the dissection of various pathways involved in various disease states. Advances in gene transfer technologies produced vectors with tissue specificity, various time courses, increasing or knocking down genes in an externally controlled fashion. To achieve this goal, appropriate vector delivery method is essential for successfully conducting experiments. In this chapter, we focus on cardiac gene transfer in rodents that can be employed for delivering both viral and nonviral vectors.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Hajjar RJ (2013) Potential of gene therapy as a treatment for heart failure. J Clin Invest 123(1):53–61. doi:10.1172/JCI62837

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Gomes AC, Falcao-Pires I, Pires AL, Bras-Silva C, Leite-Moreira AF (2013) Rodent models of heart failure: an updated review. Heart Fail Rev 18(2):219–249. doi:10.1007/s10741-012-9305-3

    Article  CAS  PubMed  Google Scholar 

  3. Gray SJ, Samulski RJ (2008) Optimizing gene delivery vectors for the treatment of heart disease. Expert Opin Biol Ther 8(7):911–922. doi:10.1517/14712598.8.7.911

    Article  CAS  PubMed  Google Scholar 

  4. Lin H, Parmacek MS, Morle G, Bolling S, Leiden JM (1990) Expression of recombinant genes in myocardium in vivo after direct injection of DNA. Circulation 82(6):2217–2221

    Article  CAS  PubMed  Google Scholar 

  5. Barr E, Carroll J, Kalynych AM, Tripathy SK, Kozarsky K, Wilson JM, Leiden JM (1994) Efficient catheter-mediated gene transfer into the heart using replication-defective adenovirus. Gene Ther 1(1):51–58

    CAS  PubMed  Google Scholar 

  6. Hajjar RJ, Schmidt U, Matsui T, Guerrero JL, Lee KH, Gwathmey JK, Dec GW, Semigran MJ, Rosenzweig A (1998) Modulation of ventricular function through gene transfer in vivo. Proc Natl Acad Sci U S A 95(9):5251–5256

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Bish LT, Morine K, Sleeper MM, Sanmiguel J, Wu D, Gao G, Wilson JM, Sweeney HL (2008) Adeno-associated virus (AAV) serotype 9 provides global cardiac gene transfer superior to AAV1, AAV6, AAV7, and AAV8 in the mouse and rat. Hum Gene Ther 19(12):1359–1368. doi:10.1089/hum.2008.123

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Turnbull IC, Eltoukhy AA, Fish KM, Nonnenmacher M, Ishikawa K, Chen J, Hajjar RJ, Anderson DG, Costa KD (2016) Myocardial delivery of lipidoid nanoparticle carrying modRNA induces rapid and transient expression. Mol Ther 24:66. doi:10.1038/mt.2015.193

    Article  CAS  PubMed  Google Scholar 

  9. Bradshaw AC, Baker AH (2013) Gene therapy for cardiovascular disease: perspectives and potential. Vascul Pharmacol 58(3):174–181. doi:10.1016/j.vph.2012.10.008

    Article  CAS  PubMed  Google Scholar 

  10. Tang T, Gao MH, Hammond HK (2012) Prospects for gene transfer for clinical heart failure. Gene Ther 19(6):606–612. doi:10.1038/gt.2012.36

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Hammoudi N, Ishikawa K, Hajjar RJ (2015) Adeno-associated virus-mediated gene therapy in cardiovascular disease. Curr Opin Cardiol 30(3):228–234. doi:10.1097/HCO.0000000000000159

    Article  PubMed  PubMed Central  Google Scholar 

  12. Di Pasquale E, Latronico MV, Jotti GS, Condorelli G (2012) Lentiviral vectors and cardiovascular diseases: a genetic tool for manipulating cardiomyocyte differentiation and function. Gene Ther 19(6):642–648. doi:10.1038/gt.2012.19

    Article  PubMed  Google Scholar 

  13. Williams PD, Kingston PA (2011) Plasmid-mediated gene therapy for cardiovascular disease. Cardiovasc Res 91(4):565–576. doi:10.1093/cvr/cvr197

    Article  CAS  PubMed  Google Scholar 

  14. Chien KR, Zangi L, Lui KO (2015) Synthetic chemically modified mRNA (modRNA): toward a new technology platform for cardiovascular biology and medicine. Cold Spring Harb Perspect Med 5(1):a014035. doi:10.1101/cshperspect.a014035

    Article  Google Scholar 

  15. Festing MF, Altman DG (2002) Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J 43(4):244–258

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This work is supported by NIH P50 HL112324, R01 HL119046, R01 HL117505, R01 HL128099, R01 HL129814, R01HL131404, & T32 HL007824 (R. J. H.), and a Transatlantic Leducq Foundation grant. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health for providing some of the gene vectors used in these studies.

Author information

Authors and Affiliations

  1. Cardiovascular Research Center, Beth Israel Deaconess Hospital, Boston, MA, USA

    Federica del Monte

  2. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA

    Kiyotake Ishikawa & Roger J. Hajjar M.D.

Authors
  1. Federica del Monte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kiyotake Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Roger J. Hajjar M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Roger J. Hajjar M.D. .

Editor information

Editors and Affiliations

  1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Kiyotake Ishikawa

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media New York

About this protocol

Cite this protocol

del Monte, F., Ishikawa, K., Hajjar, R.J. (2017). Gene Transfer to Rodent Hearts In Vivo. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 1521. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6588-5_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6588-5_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6586-1

  • Online ISBN: 978-1-4939-6588-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation