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T-Cell Differentiation pp 257–267Cite as

Developmental and Functional Assays to Study Murine and Human γδ T Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1514))

Abstract

The key roles played by gamma-delta (γδ) T cells in immunity to infection and tumors critically depend on their differentiation into effectors capable of secreting cytokines (such as interferon-γ or interleukin-17), and killing infected or transformed cells. Here we detail the main methods used to investigate the differentiation of γδ T cells from murine or human origin. We describe developmental assays, such as thymic organ cultures (TOCs) and coculture of progenitors cells with OP9-DL1 stomal cells, as well as functional assays typically employed to evaluate γδ T cell cytotoxicity and cytokine production.

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Acknowledgement

This work was supported by the European Research Council (CoG_646701 to B.S.-S.); and the Investigator FCT (to J.C.R. and K.S.) programme of Fundação para a Ciência e Tecnologia.

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Authors and Affiliations

  1. Faculdade de Medicina, Instituto de Medicina Molecular, Universidade de Lisboa, Avenida Prof. Egas Moniz, Lisbon, 1649-028, Portugal

    Julie C. Ribot, Karine Serre & Bruno Silva-Santos

Authors
  1. Julie C. Ribot
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  2. Karine Serre
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  3. Bruno Silva-Santos
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Corresponding authors

Correspondence to Julie C. Ribot or Karine Serre .

Editor information

Editors and Affiliations

  1. Laboratory of Translational Immunology, Humanitas Clinical & Research Center, Rozzano, Italy

    Enrico Lugli

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Ribot, J.C., Serre, K., Silva-Santos, B. (2017). Developmental and Functional Assays to Study Murine and Human γδ T Cells. In: Lugli, E. (eds) T-Cell Differentiation. Methods in Molecular Biology, vol 1514. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6548-9_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6548-9_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6546-5

  • Online ISBN: 978-1-4939-6548-9

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