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T-Cell Differentiation pp 127–137Cite as

Strategies for T Helper Cell Subset Differentiation from Naïve Precursors

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1514))

Abstract

In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specific polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.

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Authors and Affiliations

  1. Department of Experimental and Clinical Medicine, University of Florence and Regenerative Medicine Unit, Careggi University Hospital Florence, Viale Pieraccini 6, Florence, 50134, Italy

    Francesco Annunziato & Laura Maggi

Authors
  1. Francesco Annunziato
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  2. Laura Maggi
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Corresponding author

Correspondence to Francesco Annunziato .

Editor information

Editors and Affiliations

  1. Laboratory of Translational Immunology, Humanitas Clinical & Research Center, Rozzano, Italy

    Enrico Lugli

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Annunziato, F., Maggi, L. (2017). Strategies for T Helper Cell Subset Differentiation from Naïve Precursors. In: Lugli, E. (eds) T-Cell Differentiation. Methods in Molecular Biology, vol 1514. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6548-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6548-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6546-5

  • Online ISBN: 978-1-4939-6548-9

  • eBook Packages: Springer Protocols

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