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A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis

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Cohesin and Condensin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1515))

Abstract

In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.

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Acknowledgement

This work was supported by NSF (MCB-1121771) and NIH (GM117102).

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Authors and Affiliations

  1. Department of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA

    Jinbo Fan, Hui Jin & Hong-Guo Yu

Authors
  1. Jinbo Fan
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  2. Hui Jin
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  3. Hong-Guo Yu
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Corresponding author

Correspondence to Hong-Guo Yu .

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Editors and Affiliations

  1. Department of Biological Chemistry, School of Medicine University of California— Irvine, Irvine, California, USA

    Kyoko Yokomori

  2. Laboratory of Genome Structure and Function Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences The University of Tokyo, Tokyo, Japan

    Katsuhiko Shirahige

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Fan, J., Jin, H., Yu, HG. (2017). A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis. In: Yokomori, K., Shirahige, K. (eds) Cohesin and Condensin. Methods in Molecular Biology, vol 1515. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6545-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6545-8_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6543-4

  • Online ISBN: 978-1-4939-6545-8

  • eBook Packages: Springer Protocols

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