A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells

  • Christoph Schiklenk
  • Boryana Petrova
  • Christian H. Haering
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1515)

Abstract

Even though the formation of compact cylindrical chromosomes early during mitosis or meiosis is a prerequisite for the successful segregation of eukaryotic genomes, little is known about the molecular basis of this chromosome condensation process. Here, we describe in detail the protocol for a quantitative chromosome condensation assay in fission yeast cells, which is based on precise time-resolved measurements of the distances between two fluorescently labeled positions on the same chromosome. In combination with an automated computational analysis pipeline, this assay enables the study of various candidate proteins for their roles in regulating genome topology during cell divisions.

Key words

Chromosome segregation Genome architecture Mitosis Live cell microscopy Schizosaccharomyces pombe 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Christoph Schiklenk
    • 1
  • Boryana Petrova
    • 1
  • Christian H. Haering
    • 1
  1. 1.Cell Biology and Biophysics UnitEuropean Molecular Biology Laboratory (EMBL)69117 HeidelbergGermany

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