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Emerging Methods in Chemoproteomics with Relevance to Drug Discovery

  • Chuong Nguyen
  • Graham M. West
  • Kieran F. GeogheganEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1513)

Abstract

A powerful interplay exists between the recognition of gene families, sensitive techniques in proteomics, and the interrogation of protein function using chemical probes. The most prominent methods, such as affinity capture, activity-based protein profiling and photoaffinity labeling, are extensively reviewed in the literature. Here we briefly review additional methods developed in the past 15 years. These include “stability proteomics” methods such as proteomically analyzed cellular thermal shift assays and the use of chemical oxidation as a probe of structure, the use of multiple bead-linked kinase inhibitors to analyze inhibitor specificities, and advances in the use of proteolysis-targeting chimeras for selective protein elimination.

Key words

Chemoproteomics CETSA SPROX DARTS LiP-SRM Proteomics Kinobeads PROTAC 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Chuong Nguyen
    • 1
  • Graham M. West
    • 1
  • Kieran F. Geoghegan
    • 1
    Email author
  1. 1.Structural Biology and BiophysicsPfizer Worldwide ResearchGrotonUSA

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