Abstract
Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
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Gencay, Y.E., Birk, T., Sørensen, M.C.H., Brøndsted, L. (2017). Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni . In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_3
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DOI: https://doi.org/10.1007/978-1-4939-6536-6_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6534-2
Online ISBN: 978-1-4939-6536-6
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