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Campylobacter jejuni pp 199–210Cite as

Methods for Genome-Wide Methylome Profiling of Campylobacter jejuni

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1512))

Abstract

Methylation has a profound role in the regulation of numerous biological processes in bacteria including virulence. The study of methylation in bacteria has greatly advanced thanks to next-generation sequencing technologies. These technologies have expedited the process of uncovering unique features of many bacterial methylomes such as characterizing previously uncharacterized methyltransferases, cataloging genome-wide DNA methylations in bacteria, identifying the frequency of methylation at particular genomic loci, and revealing regulatory roles of methylation in the biology of various bacterial species. For instance, methylation has been cited as a potential source for the pathogenicity differences observed in C. jejuni strains with syntenic genomes as seen in recent publications. Here, we describe the methodology for the use of Pacific Biosciences’ single molecule real-time (SMRT) sequencing for detecting methylation patterns in C. jejuni and bioinformatics tools to profile its methylome.

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Acknowledgments

This work was supported by the Agriculture and Food Research Initiative Fellowships Grant Program of the USDA National Institute of Food and Agriculture grant 2013-67011-21155, National Institute for Allergy and Infectious Diseases grant K08AI07052303, and Iowa State University start-up funds.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA

    Kathy T. Mou

  2. Pacific Biosciences, Menlo Park, CA, USA

    Tyson A. Clark

  3. Genome Informatics Facility, Office of Biotechnology, Iowa State University, Ames, IA, USA

    Usha K. Muppirala & Andrew J. Severin

  4. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Ames, IA, 50011, USA

    Paul J. Plummer

  5. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 2426 Lloyd Vet Med Ctr., Ames, IA, 50011, USA

    Paul J. Plummer

Authors
  1. Kathy T. Mou
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  2. Tyson A. Clark
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  3. Usha K. Muppirala
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  4. Andrew J. Severin
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  5. Paul J. Plummer
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Corresponding author

Correspondence to Paul J. Plummer .

Editor information

Editors and Affiliations

  1. University of Ottawa, Ottawa, Canada

    James Butcher

  2. University of Ottawa, Ottawa, Canada

    Alain Stintzi

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Mou, K.T., Clark, T.A., Muppirala, U.K., Severin, A.J., Plummer, P.J. (2017). Methods for Genome-Wide Methylome Profiling of Campylobacter jejuni . In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6536-6_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6534-2

  • Online ISBN: 978-1-4939-6536-6

  • eBook Packages: Springer Protocols

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