Skip to main content
SpringerLink
Log in

Characterization of Ligand–Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies

  • Protocol
  • First Online:
Campylobacter jejuni

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1512))

Abstract

Chemotactic motility is an essential virulence factor for the pathogenesis of Campylobacter spp. infection. In Chapter 6, we described technologies that enable initial screening and identification of ligands able to interact with chemoreceptor sensory domains. These include amino acid and glycan arrays, NMR, and SPR that are utilized to identify potential ligands interacting with Campylobacter jejuni. Here we describe techniques that enable the characterization and evaluation of ligand–receptor binding in chemotaxis through the assessment of motility and directed chemotactic motility as well as the associated phenotypes—autoagglutination behavior, biofilm formation, ability to adhere and invade cultured mammalian cells, and colonization ability in avian hosts.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Young KT, Davis LM, Dirita VJ (2007) Campylobacter jejuni: molecular biology and pathogenesis. Nat Rev Microbiol 5(9):665–679. doi:10.1038/nrmicro1718

    Article  CAS  PubMed  Google Scholar 

  2. Zautner AE, Tareen AM, Gross U et al (2012) Chemotaxis in Campylobacter jejuni. Eur J Microbiol Immunol 2(1):24–31. doi:10.1556/EuJMI.2.2012.1.5

    Article  CAS  Google Scholar 

  3. Lertsethtakarn P, Ottemann KM, Hendrixson DR (2011) Motility and chemotaxis in Campylobacter and Helicobacter. Annu Rev Microbiol 65:389–410. doi:10.1146/annurev-micro-090110-102908

    Article  CAS  PubMed  Google Scholar 

  4. Wadhams GH, Armitage JP (2004) Making sense of it all: bacterial chemotaxis. Nat Rev Mol Cell Biol 5(12):1024–1037. doi:10.1038/nrm1524

    Article  CAS  PubMed  Google Scholar 

  5. Hendrixson DR, DiRita VJ (2004) Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract. Mol Microbiol 52(2):471–484. doi:10.1111/j.1365-2958.2004.03988.x

    Article  CAS  PubMed  Google Scholar 

  6. Yao R, Burr DH, Guerry P (1997) CheY-mediated modulation of Campylobacter jejuni virulence. Mol Microbiol 23(5):1021–1031

    Article  CAS  PubMed  Google Scholar 

  7. Takata T, Fujimoto S, Amako K (1992) Isolation of nonchemotactic mutants of Campylobacter jejuni and their colonization of the mouse intestinal tract. Infect Immun 60(9):3596–3600

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Chang C, Miller JF (2006) Campylobacter jejuni colonization of mice with limited enteric flora. Infect Immun 74(9):5261–5271. doi:10.1128/IAI.01094-05

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Hartley-Tassell LE, Shewell LK, Day CJ et al (2010) Identification and characterization of the aspartate chemosensory receptor of Campylobacter jejuni. Mol Microbiol 75(3):710–730. doi:10.1111/j.1365-2958.2009.07010.x

    Article  CAS  PubMed  Google Scholar 

  10. Ottemann KM, Miller JF (1997) Roles for motility in bacterial-host interactions. Mol Microbiol 24(6):1109–1117

    Article  CAS  PubMed  Google Scholar 

  11. Rahman H, King RM, Shewell LK et al (2014) Characterisation of a multi-ligand binding chemoreceptor CcmL (Tlp3) of Campylobacter jejuni. PLoS Pathog 10(1):e1003822. doi:10.1371/journal.ppat.1003822

    Article  PubMed  PubMed Central  Google Scholar 

  12. Golden NJ, Acheson DW (2002) Identification of motility and autoagglutination Campylobacter jejuni mutants by random transposon mutagenesis. Infect Immun 70(4):1761–1771

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Friis LM, Pin C, Pearson BM et al (2005) In vitro cell culture methods for investigating Campylobacter invasion mechanisms. J Microbiol Methods 61(2):145–160. doi:10.1016/j.mimet.2004.12.003

    Article  CAS  PubMed  Google Scholar 

  14. Hugdahl MB, Beery JT, Doyle MP (1988) Chemotactic behavior of Campylobacter jejuni. Infect Immun 56(6):1560–1566

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Kanungpean D, Kakuda T, Takai S (2011) False positive responses of Campylobacter jejuni when using the chemical-in-plug chemotaxis assay. J Vet Med Sci 73(3):389–391

    Article  PubMed  Google Scholar 

  16. Tram G, Korolik V, Day CJ (2013) MBDS solvent: an improved method for assessment of biofilms. Adv Microbiol 3(02):200

    Article  Google Scholar 

  17. Ng LK, Sherburne R, Taylor DE et al (1985) Morphological forms and viability of Campylobacter species studied by electron microscopy. J Bacteriol 164(1):338–343

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Backert S, Hofreuter D (2013) Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni. J Microbiol Methods 95(1):8–23. doi:10.1016/j.mimet.2013.06.031

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Australia

    Rebecca M. King & Victoria Korolik

Authors
  1. Rebecca M. King
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Victoria Korolik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Victoria Korolik .

Editor information

Editors and Affiliations

  1. University of Ottawa, Ottawa, Canada

    James Butcher

  2. University of Ottawa, Ottawa, Canada

    Alain Stintzi

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media New York

About this protocol

Cite this protocol

King, R.M., Korolik, V. (2017). Characterization of Ligand–Receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies. In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6536-6_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6534-2

  • Online ISBN: 978-1-4939-6536-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation