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Methods to Assess the Direct Interaction of C. jejuni with Mucins

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Campylobacter jejuni

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1512))

Abstract

Studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far Western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high-throughput manner.

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References

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Acknowledgments

This work was supported by a grant from Science Foundation Ireland (08/SRC/B1393).

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Authors and Affiliations

  1. Conway Institute, School of Medicineand Medical Science, University College Dublin, Dublin, Ireland

    Marguerite Clyne, Gina Duggan & Billy Bourke

  2. Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland

    Julie Naughton

Authors
  1. Marguerite Clyne
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  2. Gina Duggan
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  3. Julie Naughton
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  4. Billy Bourke
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Corresponding author

Correspondence to Billy Bourke .

Editor information

Editors and Affiliations

  1. University of Ottawa, Ottawa, Canada

    James Butcher

  2. University of Ottawa, Ottawa, Canada

    Alain Stintzi

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© 2017 Springer Science+Business Media New York

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Clyne, M., Duggan, G., Naughton, J., Bourke, B. (2017). Methods to Assess the Direct Interaction of C. jejuni with Mucins. In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6536-6_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6534-2

  • Online ISBN: 978-1-4939-6536-6

  • eBook Packages: Springer Protocols

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