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Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes

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Isolation of Plant Organelles and Structures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1511))

Abstract

Mitochondria are the sites of a diverse set of essential biochemical processes in plants. In order to facilitate the analysis of these functions, this chapter presents protocols for the isolation of intact mitochondria from a range of plant tissues as well two workflows for fractionation into their four subcompartments; the inner and outer membranes and the two aqueous compartments, the inter membrane space and matrix. Protocols for the assessment of mitochondrial integrity and purity through enzymatic function and suggestions of commercially available compartment marker antibodies are provided.

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Acknowledgements

This work was supported through funding by the Australian Research Council (ARC) (CE140100008). NLT (FT13010123) and AHM (FT110100242) are funded as ARC Future Fellows.

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Authors and Affiliations

  1. The ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, M316, 35 Stirling Highway, Perth, WA, 6009, Australia

    Owen Duncan

  2. The ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, M316, 35 Stirling Highway, Crawley, Perth, WA, 6009, Australia

    A. Harvey Millar

  3. The ARC Centre of Excellence in Plant Energy Biology and School of Chemistry and Biochemistry, The University of Western Australia, M316, 35 Stirling Highway, Crawley, Perth, WA, 6009, Australia

    Nicolas L. Taylor

Authors
  1. Owen Duncan
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  2. A. Harvey Millar
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  3. Nicolas L. Taylor
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Corresponding author

Correspondence to Nicolas L. Taylor .

Editor information

Editors and Affiliations

  1. The University of Western Australia, Perth, West Australia, Australia

    Nicolas L. Taylor

  2. The University of Western Australia, Perth, West Australia, Australia

    A. Harvey Millar

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Duncan, O., Millar, A.H., Taylor, N.L. (2017). Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes. In: Taylor, N., Millar, A. (eds) Isolation of Plant Organelles and Structures. Methods in Molecular Biology, vol 1511. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6533-5_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6533-5_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6531-1

  • Online ISBN: 978-1-4939-6533-5

  • eBook Packages: Springer Protocols

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