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Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP)

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HDAC/HAT Function Assessment and Inhibitor Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1510))

Abstract

Transcriptional activation by STAT5 is repressed by deacetylase inhibitors. Investigating the role of deacetylases (HDACs) in STAT5-mediated transcription implies the analysis of molecular events taking place at the chromatin level. We describe here two alternative methods of chromatin immunoprecipitation that allow the characterization of chromatin modifications ensuing STAT5 activation and its inhibition by deacetylase inhibitors, in particular changes in histone acetylation, in histone occupancy, and in the association/dissociation of transcription factors and other chromatin-associated factors.

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Acknowledgement

This work was supported by the Deutsche Forschungsgemeinschaft [RA 2010/2–1 to A.R.], the Deutsche Krebshilfe [109750 to A.R.], and institutional research funds [Foerderlinie C to A.R.; Frauenfoerderung, Bayerisches Programm zur Realisierung der Chancengleichheit fuer Frauen in Forschung und Lehre, to S.P.]. We thank Samy Unser for his permission to use part of an unpublished agarose gel picture (Fig. 1a).

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Authors and Affiliations

  1. Stat5 Signaling Research Group, Institute of Immunology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany

    Sophia Pinz & Anne Rascle

Authors
  1. Sophia Pinz
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  2. Anne Rascle
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Corresponding author

Correspondence to Anne Rascle .

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Editors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

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Pinz, S., Rascle, A. (2017). Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP). In: Krämer, O. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 1510. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6527-4_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6527-4_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6525-0

  • Online ISBN: 978-1-4939-6527-4

  • eBook Packages: Springer Protocols

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