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HDAC/HAT Function Assessment and Inhibitor Development pp 3–10Cite as

Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1510))

Abstract

Autophagy is a lysosome-dependent, intracellular pathway for the recycling of cellular components. It plays a pivotal role in both cancer development and the response to chemotherapy. Here we describe how autophagy can be monitored in living cells by flow cytometry using the cationic amphiphilic tracer dye Cyto-ID® Green. The detection of autophagy induction in the human leukemia cell line K562 after the treatment with the HDAC class I inhibitor MS-275 serves as an example for this approach.

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Acknowledgements

This study was supported by grants to OHK from the Deutsche Krebshilfe (#110909; German Cancer Aid) and the Wilhelm Sander-Stiftung (#2010.078).

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Authors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, 55131, Mainz, Germany

    Anja Göder, Nisintha Mahendrarajah & Oliver H. Krämer

Authors
  1. Anja Göder
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  2. Nisintha Mahendrarajah
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  3. Oliver H. Krämer
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Correspondence to Oliver H. Krämer .

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Editors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

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Göder, A., Mahendrarajah, N., Krämer, O.H. (2017). Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells. In: Krämer, O. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 1510. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6527-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6527-4_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6525-0

  • Online ISBN: 978-1-4939-6527-4

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