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Assessment of Basic Biological Functions Exerted by miRNAs

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MicroRNA Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1509))

Abstract

Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.

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References

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Author information

Authors and Affiliations

  1. Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland

    John Nolan, Raymond L. Stallings & Olga Piskareva

  2. National Children’s Research Centre Our Lady’s Children’s Hospital, Crumlin, Dublin, Ireland

    John Nolan, Raymond L. Stallings & Olga Piskareva

Authors
  1. John Nolan
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  2. Raymond L. Stallings
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  3. Olga Piskareva
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Corresponding author

Correspondence to Olga Piskareva .

Editor information

Editors and Affiliations

  1. Department of Science, Waterford Institute of Technology, Waterford, Ireland

    Sweta Rani

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© 2017 Springer Science+Business Media New York

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Nolan, J., Stallings, R.L., Piskareva, O. (2017). Assessment of Basic Biological Functions Exerted by miRNAs. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 1509. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6524-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6524-3_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6522-9

  • Online ISBN: 978-1-4939-6524-3

  • eBook Packages: Springer Protocols

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