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eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response

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Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1507))

Abstract

Translation initiation is the rate-limiting step of protein synthesis and highly regulated. Eukaryotic initiation factor 3 (eIF3) is the largest and most complex initiation factor consisting of 13 putative subunits. A growing number of studies suggest that eIF3 and its subunits may represent a new group of proto-oncogenes and associates with prognosis. They regulate translation of a subset of mRNAs involved in many cellular processes including proliferation, apoptosis, DNA repair, and cell cycle. Therefore, unveiling the mechanisms of eIF3 action in tumorigenesis may help identify attractive targets for cancer therapy. Here, we describe a series of methods used in the study of eIF3 function in regulating protein synthesis, tumorigenesis, and cellular response to therapeutic treatments.

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Acknowledgment

This work was supported in part by the National Natural Science Foundation of China Grants 81573463, Hunan Provincial Natural Science Foundation of China Grant 2015JJ1024, and National Institutes of Health Grant R01 CA140582.

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Authors and Affiliations

  1. Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 110 Xiang Ya Road, Changsha, Hunan, 410078, China

    Ji-Ye Yin

  2. Department of Pharmacology and Toxicology and IU Simon Cancer Center, Indiana University School of Medicine, 980 W. Walnut Street, Indianapolis, IN, 46202, USA

    Zizheng Dong & Jian-Ting Zhang

Authors
  1. Ji-Ye Yin
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  2. Zizheng Dong
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  3. Jian-Ting Zhang
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Corresponding authors

Correspondence to Ji-Ye Yin or Jian-Ting Zhang .

Editor information

Editors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA

    Narendra Wajapeyee

  2. Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA

    Romi Gupta

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Yin, JY., Dong, Z., Zhang, JT. (2017). eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response. In: Wajapeyee, N., Gupta, R. (eds) Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation. Methods in Molecular Biology, vol 1507. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6518-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6518-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6516-8

  • Online ISBN: 978-1-4939-6518-2

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