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Determination of Alternate Splicing Events Using Transcriptome Arrays

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Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1507))

Abstract

Understanding differential isoform expression is critical to mechanistically illuminate the biology underlying both normal development and disease states. High-throughput expression profiling analysis of splice variants has thus far been limited by sample requirements and an appropriate platform for quantitation and analysis. Here we describe Affymetrix GeneChip Human Transcriptome Array 2.0, which is employed for comprehensive examination of all known transcript isoforms.

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Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, School of Public Health, Cancer Research Center, Rensselaer, NY, 12144, USA

    Rebecca S. DeVaux & Sridar V. Chittur

  2. Center for Functional Genomics, University at Albany-SUNY, 1 Discovery Drive, Health Sciences Campus, Rensselaer, NY, 12144, USA

    Marcy Kuentzel, Jason Herschkowitz & Sridar V. Chittur

Authors
  1. Rebecca S. DeVaux
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  2. Marcy Kuentzel
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  3. Jason Herschkowitz
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  4. Sridar V. Chittur
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Corresponding author

Correspondence to Sridar V. Chittur .

Editor information

Editors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA

    Narendra Wajapeyee

  2. Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA

    Romi Gupta

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DeVaux, R.S., Kuentzel, M., Herschkowitz, J., Chittur, S.V. (2017). Determination of Alternate Splicing Events Using Transcriptome Arrays. In: Wajapeyee, N., Gupta, R. (eds) Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation. Methods in Molecular Biology, vol 1507. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6518-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6518-2_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6516-8

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