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Histopathology

  • Renate KainEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

The detection of fungal elements and their characterization in patient specimens provides fundamental information. On histological sections fungi are most frequently seen on skin or mucosal surfaces or as mycotic thrombi or emboli that can occlude both arteries and veins in surgical specimen from immunocompromised patients or tissues obtained from autopsies. Microbial culture continues to be the central method for diagnosing fungal infection but is complemented by histomorphology using specific stains capable of identifying previously unsuspected fungal infections or for evaluating tissue invasion. These stains employ oxidizing reagents to create aldehyde binding sites on polysaccharides (1,2-glycol groups) of fungal cell walls for either Schiff’s reagent or Silver ions. Gomori methenamine silver (GMS) and Periodic acid-Schiff (PAS) or their modifications are the most commonly used for tissue sections and in cytology specimens.

Key words

Histochemistry Schiffs’ reaction Silver stain PAS stain 

Notes

Acknowledgments

RK would like to thank Sabine Blach, BMA at the Histopathology Laboratory of the Clinical Institute of Pathology for technical advice.

References

  1. 1.
    Rotterdam H, Tsang P (1994) Gastrointestinal disease in the immunocompromised patient. Hum Pathol 25(11):1123–1140CrossRefPubMedGoogle Scholar
  2. 2.
    Bancroft JD, Gamble M (2002) Theory and practice of histological techniques, 5th edn. Churchill Livingstone, New York, NY, pp 335–338Google Scholar
  3. 3.
    Hotchkiss RD (1948) Arch Biochem 16:13Google Scholar
  4. 4.
    McManus JFA (1946) Nature 158:202CrossRefPubMedGoogle Scholar
  5. 5.
    McManus JFA (1948) Histological and histochemical uses of periodic acid. Stain Tech 23:99–108CrossRefGoogle Scholar
  6. 6.
    Carson FL (1997) Histotechnology: a self-instructional text, 2nd edn. American Society of Clinical Pathology, Chicago, ILGoogle Scholar
  7. 7.
    Gömöri G (1946) A new histochemical test for glycogen and mucin. Am J Clin Pathol 16:177CrossRefGoogle Scholar
  8. 8.
    Grocott RG (1955) A stain for fungi in tissue sections and smears using Gomori's methenamine-silver nitrate technic. Am J Clin Pathol 8(25):975–979Google Scholar
  9. 9.
    Feulgen R, Rosenbeck H. Microchemical test for nucleic acid of the thymonucleic acid type and the selective staining of cell nuclei in microscopic preparations. Z physiol Chem. 1924;135:203.Google Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Clinical Institute of PathologyMedical University of ViennaViennaAustria

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