Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue
The immune system is important to protect the host from fungal infections. Diverse cell types belonging to the innate or adaptive branch of the immune system act in a tightly coordinated and tissue-specific manner. Experimental mouse models of fungal infections have proved essential for assessing the protective principles against different fungal pathogens. Besides pathological, histological, biochemical and molecular parameters, the analysis of phenotypic and functional aspects of immune cells in infected tissues is key for understanding the mechanisms of antifungal defense. In this chapter, we describe a method based on flow cytometry to assess innate and adaptive immune cells isolated from an in vivo context in a qualitative and quantitative manner.
Key wordsCandida albicans Mouse models Host defense Innate and adaptive immunity Mucosal tissue Flow cytometry
Work in the LeibundGut-laboratory is supported by the Swiss National Science Foundation, the Gebert Rüf Foundation, the Promedica Foundation and the University of Zürich. FS is supported by an Erwin Schrödinger fellowship (J3566-B22) from the Austrian Research Fund.
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